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KMID : 0811720080120040131
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 4 p.131 ~ p.136
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Characterization of Ionic Currents in Human Neural Stem Cells
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Lim Chae-Gil
Ahn Seung-Cheol
Kim Sung-Soo
KimSuh Hae-Young
Lee Young-Don
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Abstract
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The profile of membrane currents was investigated in differentiated neuronal cells derived from human neural stem cells (hNSCs) that were obtained from aborted fetal cortex. Whole-cell voltage clamp recording revealed at least 4 different currents: a tetrodotoxin (TTX)-sensitive Na+ current, a hyperpolarization-activated inward current, and A-type and delayed rectifier-type K+ outward currents. Both types of K+ outward currents were blocked by either 5 mM tetraethylammonium (TEA) or 5 mM 4-aminopyridine (4-AP). The hyperpolarization-activated current resembled the classical K+ inward current in that it exhibited a voltage-dependent block in the presence of external Ba2+ (30?M) or Cs+ (3?M). However, the reversal potentials did not match well with the predicted K+ equilibrium potentials, suggesting that it was not a classical K+ inward rectifier current. The other Na+ inward current resembled the classical Na+ current observed in pharmacological studies. The expression of these channels may contribute to generation and repolarization of action potential and might be regarded as functional markers for hNSCs-derived neurons.
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KEYWORD
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Human neural stem cells, TTX-sensitive Na+ current, A-type, delayed rectifier, hyperpolarization-activated inward current
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